期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：11
页码：2810-2813
DOI：10.1073/pnas.68.11.2810
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The spleen from a patient with adult Gaucher's disease was shown to be deficient in a {beta}-glucosidase (EC 3.2.1.21 ) isoenzyme that has optimal activity at pH 4.0-4.3, and is stimulated by 0.02% Triton X-100. A mixture of spleen homogenates from a control and from the patient contained {beta}-glucosidase activity equivalent to 2-3 times the theoretical expected activity. The increase in enzyme activity occurred at pH 4.0-4.3; the magnitude of the increase was proportional to the amount of each homogenate added. Two factors, one called factor P from the patient's spleen, the other called factor C from the control spleen, were responsible for a reconstitution of {beta}-glucosidase activity in vitro. Factor P is tentatively identified as an acid glycoprotein.
