期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：11
页码：2769-2773
DOI：10.1073/pnas.68.11.2769
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：During the amplification stage in ovaries, the complete repetitive unit of the DNA that codes for ribosomal RNA in Xenopus appears to be transcribed. This large RNA transcript is found in a complex with DNA. Substitution experiments with 5-bromodeoxyuridine do not show any evidence that a complete amplified cistron is used as a template for further amplification. A derivative of rifampicin, 2',5'-dimethyl-N(4')benzyl-N(4')[desmethyl] rifampicin, preferentially inhibits the DNA synthesis responsible for ribosomal gene amplification. These results are consistent with the hypothesis that RNA-dependent DNA synthesis is involved in gene amplification.
关键词：density gradient centrifugation ; 2′,5′-dimethyl- N (4′)benzyl- N (4′)[desmethyl] rifampicin ; extrachromosomal DNA ; Xenopus laevis ; ovaries
