期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：12
页码：2913-2917
DOI：10.1073/pnas.68.12.2913
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 x 105 to 7.4 x 104 daltons, as determined by electron microscopy, DNA content, or electrophoretic mobility.
关键词：gel electrophoresis ; electron microscopy ; DNA mapping ; DNA fragments ; tumor virus
