期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：12
页码：2932-2936
DOI：10.1073/pnas.68.12.2932
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：50S ribosomal subunits from Bacillus stearothermophilus can be reconstituted from their dissociated components, namely a 5S RNA-free protein fraction, a 5S RNA-free 23S ribosomal RNA fraction, and purified 5S RNA. The biological activity of reconstituted particles in polypeptide synthesis is dependent on the presence of 5S RNA. In the absence of 5S RNA, particles are produced that have greatly reduced activity in (a) polypeptide synthesis directed by synthetic, as well as natural, messenger RNA, (b) peptidyl transferase assay, (c) [3H]UAA binding dependent on peptide chain termination factor R1, (d) G factor-dependent [3H]GTP binding, and (e) codon-directed tRNA binding assayed in the presence of 30S subunits. Thus, 5S RNA is an essential 50S ribosomal component.
关键词：reconstitution ; B. stearothermophilus ; termination ; peptidyl transferase ; tRNA binding ; G factor
