期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：12
页码：2954-2957
DOI：10.1073/pnas.68.12.2954
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In Escherichia coli mutants deficient in DNA polymerase I, newly replicated short DNA is joined at about 10% of the rate in the wild-type strains. It is postulated that DNA polymerase I normally functions in filling gaps between the nascent short segments synthesized by the replication complex. Possible implications of the finding are discussed in relation to other abnormal properties of these mutants.
关键词：DNA biosynthesis ; discontinuous replication ; pulse labeling ; density gradient centrifugation ; DNA polymerase
