标题：Enzymatic Conversion of 5-Oxo-L-Proline (L-Pyrrolidone Carboxylate) to L-Glutamate Coupled with Cleavage of Adenosine Triphosphate to Adenosine Diphosphate, a Reaction in the γ-Glutamyl Cycle
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：12
页码：2982-2985
DOI：10.1073/pnas.68.12.2982
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A new enzyme, 5-oxoprolinase, was found in rat kidney and in several other tissues; it catalyzes the conversion of 5-oxo-L-proline (L-5-oxo-pyrrolidine-2-carboxylic acid, L-2-pyrrolidone-5-carboxylic acid, L-pyroglutamic acid) to L-glutamic acid, with concomitant stoichiometric cleavage of ATP to ADP and orthophosphate. The reaction catalyzed by 5-oxoprolinase, in which 5-oxoproline formed from {gamma}-glutamyl amino acids by the action of {gamma}-glutamylcyclotransferase is converted to glutamate, appears to function in the {gamma}-glutamyl cycle. 5-Oxoprolinase requires Mg++ (or Mn++) and K+ (or NH4+) for activity. The equilibrium is markedly in favor of glutamate formation at pH 7.8.
关键词：kidney ; amino-acid transport ; pyroglutamic acid ; 5-oxo-pyrrolidine-2-carboxylic acid
