标题：A Transport Form of Collagen from Embryonic Tendon: Electron Microscopic Demonstration of an NH2-Terminal Extension and Evidence Suggesting the Presence of Cystine in the Molecule
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：1
页码：60-64
DOI：10.1073/pnas.69.1.60
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：When cells were isolated from chickembryo tendons and incubated in vitro for 2-6 hr, essentially all the newly-synthesized collagen was recovered from the incubation medium as a transport form larger than tropocollagen. Experiments in which cells were incubated with [14C]cystine suggested that the transport form contained cystine and that it was, in part, stabilized by disulfide bonds. Electron microscopy of segment-long-spacing aggregates prepared from the transport form of collagen showed that the native molecule differed from tropocollagen in that it had an extension of about 13 nm (130 A) at the NH2-terminal end.
