文章基本信息

标题：Nucleotide Sequencing of DNA: Preliminary Characterization of the Products of Specific Cleavages at Guanine, Cytosine, or Adenine Residues
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作者：Winston Salser ; Kirk Fry ; Clifford Brunk 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：1
页码：238-242
DOI：10.1073/pnas.69.1.238
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：DNA synthesized in vitro from a phage M13 template has been cleaved at either guanine, adenine, or cytosine residues by ribosubstitution techniques. Fingerprints of the fragments obtained suggest that DNA sequencing will be possible with this technique.
关键词：bacteriophage M13 ; ribosubstitution ; DNA polymerase I ; electrophoresis ; two-dimensional fingerprinting