期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：2
页码：371-373
DOI：10.1073/pnas.69.2.371
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have developed a new micromethod for testing phytohemagglutinin stimulation of human lymphocytes. 50 {micro}l of peripheral blood is incubated at 37{degrees}C directly, without cell separation, with 50 {micro}l of tissue culture medium containing 50{micro}g of phytohemagglutinin for 24 hr. Then, 0.5 {micro}Ci of tritiated thymidine in 50 {micro}l of tissue culture medium is added and incubated for an additional 16 hr. After incubation, erythrocytes are selectively lysed in distilled water, and white blood cells are trapped quickly on a Millipore filter. The radioactivity of the filter is then assessed by a scintillation counter. The requirement of extremely small amounts of blood and a short incubation time are advantages. Further, this method provides a most useful evaluation of thymusdependent cell populations in man and experimental animals and, for the first time, this method provides the possibility of quantitating the deficiency of thymus-dependent cells.
