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  • 标题:Nature of Oxygen Inhibition of Nitrogenase from Azotobacter vinelandii
  • 本地全文:下载
  • 作者:Peter P. Wong ; R. H. Burris
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1972
  • 卷号:69
  • 期号:3
  • 页码:672-675
  • DOI:10.1073/pnas.69.3.672
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The reduction of nitrogen, acetylene, azide, and cyanide at various oxygen concentrations by nitrogenase from Azotobacter vinelandii was measured with a well-defined system. Oxygen inhibited the reduction of each substrate uncompetitively. The inhibition constants (Ki) were 0.014, 0.023, 0.008, and 0.003 atm of oxygen for reduction of nitrogen, acetylene, azide, and cyanide, respectively. The system used included ATP-generating components, subcellular particles from A. vinelandii with high nitrogenase specific activity, and illuminated spinach chloroplasts plus carriers to supply electrons. Oxygen did not affect the photochemical electron donating system, but it did inhibit nitrogenase-dependent ATP hydrolysis.
  • 关键词:kinetics ; oxygen ; uncompetitive inhibition
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