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  • 标题:Characterization of Messenger Ribonucleoprotein and Messenger RNA from KB Cells
  • 本地全文:下载
  • 作者:Ajit Kumar ; Uno Lindberg
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1972
  • 卷号:69
  • 期号:3
  • 页码:681-685
  • DOI:10.1073/pnas.69.3.681
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Messenger ribonucleoprotein and mRNA from KB-cells were isolated under conditions designed to minimize nonspecific RNA-protein interaction and to minimize degradation by contaminating ribonucleases. A large fraction, 60-70%, of the messenger ribonucleoprotein from polysomes dissociated in vitro by either EDTA or puromycin sedimented faster than the large ribosome subunit. Messenger ribonucleoprotein particles with sedimentation coefficients up to 200 S were observed. Released mRNA was also large, with maximal molecular weights around 5 x 106.
  • 关键词:eukaryotic cells ; polysomes ; molecular weight ; density gradient centrifugation
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