期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：5
页码：1176-1180
DOI：10.1073/pnas.69.5.1176
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The RNA isolated from avian myeloblastosis virus was fractionated by sucrose density gradient centrifugation. The 3'-OH terminal nucleosides of various fractions were determined by periodate oxidation followed by tritiated borohydride reduction. The 60-70S fraction and the 35S RNA derived from it by heating both have adenosine as the major terminal nucleoside, with cytidine as the next most frequent terminal. Control samples of tRNAmetf. coli and 28S ribosomal RNA from mouse ascites tumor cells gave the expected terminal residues and molecular weights.
关键词：sucrose density centrifugation ; [3H]borohydride reduction ; 60-70S RNA
