期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：5
页码：1249-1252
DOI：10.1073/pnas.69.5.1249
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Evidence for two species of elongation factor 1 (EF 1A and EF 1B) from calf brain has been obtained by molecular sieve chromatography on Sephadex G-150. A high molecular weight form, EF 1A, interacts with GTP to form an EF 1A-GTP complex. GDP also reacts with EF 1, but unlike the reaction with GTP, an EF 1B-GDP complex is formed that contains a lower molecular weight and labile species of EF 1. The results also indicate that EF 1A-GTP reacts with aminoacyl-tRNA to form an aminoacyl-tRNA-EF 1B-GTP complex. These results are discussed with regard to the role of EF 1 in aminoacyl-tRNA binding to ribosomes.
