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  • 标题:Fluorescence of Lysozyme: Emissions from Tryptophan Residues 62 and 108 and Energy Migration
  • 本地全文:下载
  • 作者:T. Imoto ; Leslie S. Forster ; J. A. Rupley
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1972
  • 卷号:69
  • 期号:5
  • 页码:1151-1155
  • DOI:10.1073/pnas.69.5.1151
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The bulk of the fluorescence of lysozyme is located in Trp 62 and Trp 108. By examination of the fluorescence of derivatives in which Trp 62 and/or Trp 108 are specifically oxidized, it has been possible to detect a pH-dependent interaction between tryptophan residues. This interaction is interpreted as energy transfer from Trp 108 to Trp 62.
  • 关键词:pH dependence ; spectroscopy ; protein structure ; enzyme-substrate complex
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