期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：6
页码：1425-1428
DOI：10.1073/pnas.69.6.1425
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：10S RNA has been isolated from the postribosomal supernatant of rabbit reticulocyte lysate. This RNA is associated with protein in a ribonucleoprotein particle that sediments at approximately 20S. The 10S RNA extracted from this particle codes predominantly for [α] globin chains when it is translated by a mouse ascites cell-free extract. The product of the cell-free synthesis has been characterized by column chromatography and electrophoresis of the tryptic peptides. About 20% of the total [α] chain mRNA is present in the postribosomal supernatant, whereas {beta} chain mRNA is almost exclusively associated with polyribosomes.
