期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：7
页码：1877-1882
DOI：10.1073/pnas.69.7.1877
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Poliovirus RNA that has been derivatized at the 3'-end with NaIO4-NaB3H4 yields, after hydrolysis with alkali or RNase T2, predominantly labeled residues of modified adenosine; no labeled nucleoside derivative is produced by digestion with RNase A or RNase T1. The 3'-terminal bases of the RNA are, therefore...ApAOH. Hydrolyzates of poliovirus [32P]RNA, after exhaustive digestion with RNase T1 or RNase A, contain, besides internal oligonucleotides, polynucleotides resistant to further action of ribonucleases T1 and A, respectively; these polynucleotides were isolated by membrane-filter binding or ion-exchange chromatography. The sequence of the T1-resistant polynucleotide was determined to be (Ap)nAOH, that of the RNase A-resistant polynucleotide was GpGp(Ap)nAOH. The chain length (n) of the polyadenylic acid, as analyzed by different methods, averages 89 nucleotides. Gel electrophoresis revealed heterogeneity of the size of poly(A). Poliovirus RNA, when labeled in vitro at the 3'-end, contains [3'-3H]poly(A); when labeled in vivo with [3H]A, it contains [3H](Ap)nAOH. The data establish that... YpGpGp(Ap)[unk]AOH is the 3'-terminal sequence of poliovirus RNA, Type 1 (Mahoney). Since this mammalian virus reproduces in the cell cytoplasm, these observations may modify prior interpretations of the function of polyadenylate ends on messenger RNAs.
