期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：8
页码：2155-2159
DOI：10.1073/pnas.69.8.2155
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A synthetic polymer, polyethyleneimine containing dodecyl (C12H25)-chains for binding sites and imidazole moieties for functional catalytic groups, catalyzes the hydrolysis of phenolic sulfate esters by a two-step mechanism resembling that of the corresponding natural enzyme. Quantitative rate studies give kinetic parameters that show that the polymer (synzyme) accelerates the rate 1012-fold compared to unbound imidazole, and 102-fold compared to a type IIA aryl sulfatase enzyme.
