期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：8
页码：2273-2277
DOI：10.1073/pnas.69.8.2273
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：When transfer of electronic excitation energy occurs between a donor-acceptor pair by the Forster mechanism, the decay of fluorescence of the donor follows first-order kinetics, with a rate constant that depends on the distance from donor to acceptor. In a system that contains donor-acceptor pairs of different separations, the fluorescence decay of the donors will not be exponential, but will depend on the distribution function of donor-acceptor distances, f(r). Various approaches are outlined for the extraction of information about f(r) from the decay curve of donor fluorescence. Specifically, if a plausible expression with adjustable parameters is assumed for f(r), numerical methods can be used to evaluate the parameters that yield the closest fit between the observed decay curve and that calculated from the assumed f(r). The technique of fluorescence decay may prove to be useful for determination of distribution functions of end-to-end distances of polymers to the edges of which suitable donor-acceptor chromophore pairs have been attached.
关键词：energy transfer ; fluorescence ; decay ; conformation ; polymers
