期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：8
页码：2095-2099
DOI：10.1073/pnas.69.8.2095
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：7-9S RNA has been isolated from polyribosomes of HeLa S-3 cells in S phase. These RNA species direct the synthesis in vitro of all five classes of human histones when they are added to a protein-synthesizing system derived from mouse ascites tumor cells. The histones synthesized in vitro have been identified by two kinds of acrylamide gel electrophoresis, and by paper electrophoresis of tryptic digests. By use of the ratio of tyrosine to tryptophan incorporation as an index of contamination, it was determined that less than 10% of the 7-9S RNA is translated into proteins other than histones.
关键词：7-9S RNA ; histones ; protein synthesis ; acrylamide gel electrophoresis
