期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：10
页码：2885-2889
DOI：10.1073/pnas.69.10.2885
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Four clonal lines have been established in tissue culture from a transplantable tumor of cervical nerve-root induced transplacentally with ethylnitrosourea in an inbred BD-IX rat strain. One clone (RN-2) has several biochemical properties of the nervous system that led to its classification as a Schwann cell line, namely (a) the synthesis of the nervous system specific protein S-100, (b) a high specific activity of 2',3'-cyclic nucleotide-3'-phosphohydrolase, an enzyme present in high specific activity only in glial cells, and (c) the presence of a basic protein related by immunological crossreaction, molecular size, and amino-acid composition to the encephalitogenic protein from beef brain myelin. Clone RN-2 has a high plating efficiency, a doubling time of about 20 hr, and a mean of 43 chromosomes of normal morphology. Clone RN-2 has also been inoculated subcutaneously into syngeneic rats and grown as a clonal tumor with good growth properties.
关键词：S-100 protein ; 2′,3′-cyclic nucleotide-3′-phosphohydrolase ; myelin basic protein ; peripheral nervous system
