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  • 标题:Enhancement of RNA Polymerase Activity by a Factor Released by Auxin from Plasma Membrane
  • 本地全文:下载
  • 作者:James W. Hardin ; Joe H. Cherry ; D. James Morré
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1972
  • 卷号:69
  • 期号:11
  • 页码:3146-3150
  • DOI:10.1073/pnas.69.11.3146
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Using recently developed techniques for solubilization of RNA polymerase from soybean chromatin and isolation of plasma membrane fractions from plants we can show the presence of a transcriptional factor specifically released from the membranes by auxin, 2,4-dichlorophenoxyacetic acid. The nonauxin, 3,5-dichlorophenoxyacetic acid, does not release the factor, but subsequent exposure of the membranes to auxin results in its release. Factor activity could not be demonstrated in fractions devoid of plasma membranes. The presence of a regulatory factor for RNA polymerase associated with plant plasma membrane and specifically released by auxin provides a mechanism whereby both rapid growth responses and delayed nuclear changes could be derived from a common auxin receptor site associated with plasma membrane.
  • 关键词:soybean ; transcription factor ; chromatin
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