期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：12
页码：3606-3610
DOI：10.1073/pnas.69.12.3606
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Positive control in vitro by gene N protein of bacteriophage {lambda} was demonstrated. {lambda} DNA was used to direct in vitro synthesis of {lambda} endolysin in a cell-free protein-synthesizing preparation derived from Escherichia coli. The endolysin synthesis depends on the concomitant in vitro synthesis of {lambda} gene N protein. When {lambda} N- DNA was used to direct the cell-free preparation, endolysin was made only if extract was added from cells in which a {lambda} prophage had been induced. The use of various prophage deletion strains proved that if this stimulating activity made in vivo is coded by a known {lambda} gene, it must be coded by gene N. The ability to stimulate endolysin synthesis in vitro on a {lambda} N- DNA template, therefore, constitutes an assay for N protein.
关键词：N protein assay ; deletion mapping ; N protein synthesis
