期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:1972
卷号:69
期号:12
页码:3615-3619
DOI:10.1073/pnas.69.12.3615
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:A cell-free extract from cultured rat hepatoma cells is described that incorporates amino acids into chains of tyrosine aminotransferase that are identical, by several criteria, with tyrosine aminotransferase from rat liver. Extracts from steroid-induced cells are 6-to 10-times more active for tyrosine aminotransferase synthesis than extracts from uninduced cells, although the total incorporation rates are identical.
关键词:rat ; immunoprecipitation ; protein purification ; steroid ; hormone