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  • 标题:Synthesis of Inducible Tyrosine Aminotransferase in a Cell-Free Extract from Cultured Hepatoma Cells
  • 本地全文:下载
  • 作者:Jean-Paul Beck ; Giselle Beck ; K. Y. Wong
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1972
  • 卷号:69
  • 期号:12
  • 页码:3615-3619
  • DOI:10.1073/pnas.69.12.3615
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:A cell-free extract from cultured rat hepatoma cells is described that incorporates amino acids into chains of tyrosine aminotransferase that are identical, by several criteria, with tyrosine aminotransferase from rat liver. Extracts from steroid-induced cells are 6-to 10-times more active for tyrosine aminotransferase synthesis than extracts from uninduced cells, although the total incorporation rates are identical.
  • 关键词:rat ; immunoprecipitation ; protein purification ; steroid ; hormone
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