期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：12
页码：3703-3707
DOI：10.1073/pnas.69.12.3703
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Bacteriophage T4 induces the synthesis of eight transfer RNAs upon infection of E. coli. The tRNAs are easily detected and resolved into pure species by polyacrylamide gel electrophoresis of RNA labeled with 32P after T4 infection. Two-dimensional fingerprints of RNase T1 products derived from individual gel bands give patterns characteristic of single tRNAs. Furthermore, the T1 digest of each gel band has a single oligonucleotide that contains the minor nucleotides Tp and {Psi}p, a characteristic feature of all known tRNAs. Four larger RNAs are also seen in the polyacrylamide gels. Fingerprint and genetic analyses demonstrate that these molecules are related to the tRNAs, but the exact nature of this relationship is not known.
关键词：RNA sequence analysis ; gel electrophoresis ; biosynthesis of tRNA ; deletion mutation
