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  • 标题:Binding of Thymus- and Bone Marrow-Derived Lymphoid Cells to Antigen-Derivatized Fibers
  • 本地全文:下载
  • 作者:U. Rutishauser ; G. M. Edelman
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1972
  • 卷号:69
  • 期号:12
  • 页码:3774-3778
  • DOI:10.1073/pnas.69.12.3774
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Thymus-derived lymphocytes (T cells) and bone marrow-derived lymphocytes (B cells) from mouse spleens bind specifically to antigen-derivatized nylon fibers. The fiber-bound population consisted of about 60%-70% B cells and 30% T cells as determined by cytotoxicity, fluorescence, and antibody-complement binding assays. Essentially all fiber-bound cells were viable and could be accounted for as T or B cells. Enriched populations of T or B cells could be isolated on the fibers by destruction of one or the other cell type with the appropriate antiserum plus complement. T or B cells could also be fractionated according to their relative affinity (or avidity) for a given antigen.
  • 关键词:mouse spleen ; nylon fibers ; antiserum ; cytotoxicity ; fluorescence
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