期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：12
页码：3815-3819
DOI：10.1073/pnas.69.12.3815
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A polycytidylate-dependent RNA polymerase of encephalomyocarditis virus was isolated from infected BHK 21 cells. The enzyme was associated with a smooth-membrane fraction, from which it was extracted by a mixture of sodium dodecyl sulfate, Triton X-100, and dithiothreitol, and further purified by chromatography on a Dowex-1 column and by glycerol gradient sedimentation. Analysis of a 6S glycerol gradient peak of RNA polymerase activity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of five polypeptides, of molecular weights 72,000, 65,000, 57,000, 45,000, and 35,000. The molecular weights of four of the polypeptides (72,000, 65,000, 45,000, and 35,000) are almost identical to the reported molecular weights of the four subunits of Q{beta} replicase.
关键词：BHK 21 cells ; smooth membranes ; electron microscopy
