期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：1
页码：129-133
DOI：10.1073/pnas.70.1.129
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Ultraviolet (UV)-stimulated nonconservative DNA synthesis has been observed in E. coli rendered permeable to nucleoside triphosphates by exposure to toluene. This synthesis is detected in toluenized cells in which the background of UV-independent DNA synthesis is reduced by use of dnaB mutants temperature-sensitive for DNA replication and additionally deficient in DNA polymerase I. UV-stimulated nonconservative synthesis is also seen in polA dnaE mutants that are deficient in two of the three known DNA polymerases. The observed UV-stimulated repair-like synthesis requires the presence of the four deoxyribonucleoside triphosphates and ATP. This mode of synthesis appears to differ from the ATP-independent nonconservative DNA synthesis previously reported to occur in toluenized bacteria.
