期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：1
页码：200-204
DOI：10.1073/pnas.70.1.200
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The DNA of adenovirus type 2 was cleaved by restriction endonuclease R{middle dot}RI into six fragments. These fragments were separated by electrophoresis on composite agarose-polyacrylamide gels. Their molecular weights ranged from 1.1 x 106 to 13.6 x 106, as measured by electron microscopy. Each fragment represented a unique segment of adenovirus type 2 DNA since: (i) the fragments were obtained in equimolar amounts; (ii) the sum of their molecular weights was equal to the molecular weight of complete adenovirus DNA; and (iii) each fragment exhibited a rate of renaturation that was inversely proportional to its size.
关键词：restriction enzyme ; electron microscopy ; DNA gel electrophoresis ; DNA sequence complexity
