期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：2
页码：294-297
DOI：10.1073/pnas.70.2.294
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Electron paramagnetic resonance (EPR) spectroscopy has revealed a new free-radical signal produced by illumination of spinach chloroplasts at 77{degrees}K. This signal is observed only when an oxidant (ferricyanide) is added to the chloroplast suspension in the dark before illumination. The EPR signal is produced at 77{degrees}K by illumination with 645-nm monochromatic light capable of activating Photosystem II but not with 715-nm illumination capable of activating Photosystem I. Furthermore, since the signal shows a relative increase in chloroplast fragments enriched in Photosystem II but is absent in chloroplast fragments enriched in Photosystem I, we conclude that this new EPR signal is associated with the primary photoact of Photosystem II in chloroplasts. On the basis of the measured EPR parameters (g = 2.0026 {+/-} 0.0002, linewidth = 8 G), it is suggested that the signal may be associated with the reaction-center chlorophyll of Photosystem II.
关键词：photosynthesis ; electron transfer ; light reactions ; electron paramagnetic resonance
