期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：2
页码：421-424
DOI：10.1073/pnas.70.2.421
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A quantitative assay for the N protein of bacteriophage {lambda} has been used to study the in vivo regulation of N gene expression. The assay makes use of the observation that in a cell-free protein-synthesizing system from Escherichia coli programmed with {lambda}N- DNA the {lambda} endolysin is made only if N protein is added to the reaction. The rate of synthesis of N protein in vivo is negatively controlled by the products of the CI and tof genes of the phage. Furthermore, N protein activity is extremely unstable in vivo. During normal cell growth at 35{degrees
关键词：N protein assay ; in vitro endolysin synthesis ; tof gene control ; functionally unstable protein
