期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：2
页码：315-319
DOI：10.1073/pnas.70.2.315
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Cytoplasmic extracts from Krebs II mouse ascites cells and from L cells translate messenger RNA from coliphage Q{beta} with fidelity to produce products that migrate on polyacrylamide gels with those products directed by Q{beta} RNA in an Escherichia coli cell-free system. The mammalian cell extracts correctly initiate and terminate Q{beta} coat protein synthesis, as shown by: (i) [3H]lysine-and [3H]arginine-labeled tryptic peptides derived from the coat-sized product resemble these from authentic Q{beta} coat protein, (ii) Q{beta} coat (which contains methionine only at the N-terminal end) can be radioactively labeled with methionine only if the methionine is formylated, and (iii) L cell extracts directed by Q{beta} am-11 (an amber mutant in the coat protein) RNA make no completed coat-sized material, but do make a peptide the size of the authentic amber coat fragment.
关键词：L cells ; Krebs ascites cells ; coat protein ; tryptic peptides ; N -formylmethionine
