期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：3
页码：941-943
DOI：10.1073/pnas.70.3.941
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Flavin 1,N6-ethenoadenine dinucleotide ([epsilon] FAD) was prepared by the action of chloroacetaldehyde on flavin adenine dinucleotide. This compound, which has two potential fluorophores, [epsilon] -adenine and isoalloxazine, shows extremely efficient energy transfer from the former to the latter. The fluorescences of both moieties are greatly diminished in the intact molecule. Determination of the fluorescence yields and lifetimes leads to the conclusion that at 20 degrees in neutral aqueous solution [epsilon] FAD exists mainly (90%) as an internally complexed or stacked form.
关键词：FAD ; 1, N 6-ethenoadenine derivative ; fluorescence emission ; coenzyme analogue ; energy transfer
