期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：4
页码：1046-1049
DOI：10.1073/pnas.70.4.1046
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Analyses were performed on the purified initiator tRNA from rabbit liver to test for the presence of {Psi}p and Tp in this molecule. Neither of these nucleotides could be detected after hydrolysis by piperidine, NaOH, or T2 RNase. Similarly digestion with venom phosphodiesterase plus phosphatase failed to release any pseudourdine or ribothymidine. Identical results were obtained with the initiator tRNA from sheep mammary gland. The absence of these nucleosides was confirmed by pancreatic and T1 RNase digestion of the rabbit-liver initiator tRNA. The classical G-T-{Psi}-C sequence was not detected in this molecule. An A-U-C-G sequence has been identified; it may possibly replace the G-T-{Psi}-C sequence.
