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  • 标题:Isolation of Messenger RNA Coding for Mouse Heavy-Chain Immunoglobulin
  • 本地全文:下载
  • 作者:R. H. Stevens ; A. R. Williamson
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1973
  • 卷号:70
  • 期号:4
  • 页码:1127-1131
  • DOI:10.1073/pnas.70.4.1127
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:A method for purification of the mRNA coding for heavy-chain protein of a mouse G2a myeloma (5563) is reported. Complete myeloma protein (H2L2) specifically binds to the heavy-chain mRNA, and the resultant RNA-protein complex is precipitated with antiserum against 5563 myeloma protein. Cytoplasmic RNA isolated by this method showed two bands of 6.0 x 105 and 3.1 x 105 molecular weight when analyzed by acrylamide gel electrophoresis. The RNA in the bands contained 1.5% and 8% A-rich regions, respectively. The RNA in each of the bands contained sequences for heavy-chain protein when translated in Xenopus laevis oocytes.
  • 关键词:myeloma ; Xenopus laevis oocyte injection ; antiserum ; acrylamide gel electrophoresis
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