期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:1973
卷号:70
期号:4
页码:1127-1131
DOI:10.1073/pnas.70.4.1127
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:A method for purification of the mRNA coding for heavy-chain protein of a mouse G2a myeloma (5563) is reported. Complete myeloma protein (H2L2) specifically binds to the heavy-chain mRNA, and the resultant RNA-protein complex is precipitated with antiserum against 5563 myeloma protein. Cytoplasmic RNA isolated by this method showed two bands of 6.0 x 105 and 3.1 x 105 molecular weight when analyzed by acrylamide gel electrophoresis. The RNA in the bands contained 1.5% and 8% A-rich regions, respectively. The RNA in each of the bands contained sequences for heavy-chain protein when translated in Xenopus laevis oocytes.