期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：4
页码：1069-1072
DOI：10.1073/pnas.70.4.1069
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A crude membrane fraction was isolated from both killer M(k) and isogenic nonkiller M(o) strains of yeast labeled with [3H]adenine. Nucleic acids were extracted from this fraction and centrifuged to equilibrium in an ethidium bromide-Cs2SO4 solution. A peak of radioactivity coincident with a single fluorescent band was present in the membrane fraction isolated from cells of the killer strain but was absent from that isolated from nonkiller cells. This material has been characterized as double-stranded RNA by treatment with various nucleases and by chromatography on a cellulose CF-11 column. Sedimentation in a sucrose gradient indicates an S value of 8-10.
