期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：5
页码：1554-1558
DOI：10.1073/pnas.70.5.1554
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Treatment of synchronous cultures of Chlamydomonas reinhardtii with chloramphenicol at 4 hr after the beginning of the light phase led to a preferential loss of 70S ribosomes from the 17,000 x gmax supernate. The "lost" 70S ribosomes were found associated with a thylakoid membrane fraction prepared from the 17,000 x gmax pellet. Electron microscopic examinations of this fraction revealed that the 70S ribosomes were bound to the unstacked regions of the thylakoid membranes as polygonal penta- and hexamers. These bound ribosomes were only released by treatment with 500 mM KCl and puromycin, suggesting that both ionic interactions and nascent peptide chains were involved in the ribosome-membrane attachment. Since growth of the thylakoid membranes occurs in the light, it is suggested that bound chloroplast ribosomes function in the synthesis of thylakoid membrane proteins.
关键词：chloramphenicol ; puromycin reaction ; electron microscopy ; synchronous cultures
