期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：5
页码：1574-1578
DOI：10.1073/pnas.70.5.1574
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A particulate fraction not present in wild-type cells has been isolated from the cytoplasm and from lysed mitochondria of the respiratory-deficient extranuclear mutant "abnormal-1" of Neurospora crassa. The particles have a density between 1.13 and 1.2 g/ml. They appear in thin sections after OsO4 fixation as virus-like polymorphic vesicles containing an electron-dense nucleoid of 120-170 nm in diameter. The central core is surrounded by one or two "unit membrane" envelopes of 100 A thickness. The particles contain a single-stranded 33S RNA that is converted to 7-9S RNA by heat treatment in the presence of sodium dodecyl sulfate and that differs in its base composition from mitochondrial and cytoplasmic ribosomal RNA. They contain 7% phospholipids rich in phosphatidylethanolamine and two major proteins, a lipoprotein of molecular weight 15,000 and a glycoprotein of molecular weight 95,000. It is suggested that these virus-like particles originate within mitochondria.
