期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：6
页码：1708-1712
DOI：10.1073/pnas.70.6.1708
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The evolution of the cholinergic (nicotinic) receptor in chick muscles is monitored during embryonic development with a tritiated [α]-neurotoxin from Naja nigricollis and compared with the appearance of acetylcholinesterase. The specific activity of these two proteins reaches a maximum around the 12th day of incubation. By contrast, choline acetyltransferase reaches an early maximum of specific activity around the 7th day of development, and later continuously increases until hatching. Injection of [α]-toxin in the yolk sac at early stages of development causes an atrophy of skeletal and extrinsic ocular-muscles and of their innervation. In 16-day embryos treated by the [α]-toxin, the endplates revealed by the Koelle reaction are almost completely absent. The total content and specific activities of acetylcholinesterase and choline acetyltransferase in atrophic muscles are markedly reduced.
关键词：cholinergic receptor ; choline acetyltransferase ; acetylcholinesterase ; embryonic development of neuromuscular junction
