期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：6
页码：1860-1864
DOI：10.1073/pnas.70.6.1860
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Carrier-free iodination of 5S RNA with 125I yields a probe suitable for use in cytological RNA-DNA hybridization studies. The genes coding for 5S ribosomal RNA in Drosophila could be localized by autoradiography after a 2-day exposure, whereas a 2-month exposure is needed when the best available [3H]RNA is used. The procedure introduces one covalently bound 125I atom per 100 nucleotides, resulting in a specific activity of over 108 dpm/{micro}g of RNA. The method may be readily applied to other problems involving molecular hybridization techniques.
