期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：8
页码：2189-2192
DOI：10.1073/pnas.70.8.2189
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：HeLa cell nuclear DNA contains an unexpectedly large amount of long pyrimidine polynucleotides. These sequences were detected in DNA labeled with [3H] thymidine by treatment with formic acid-diphenylamine and subsequent analysis by polyacrylamide gel electrophoresis. About 0.5% of the total thymine residues were found in polynucleotides that migrated more slowly than 4S RNA. No polynucleotides of comparable size were detected in Escherichia coli DNA. The pyrimidine polynucleotides contained no pure poly(dT) sequences (less than 0.0015% of the total residues), as judged by thermal chromatography of a complex formed with poly(A).
