期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：8
页码：2193-2195
DOI：10.1073/pnas.70.8.2193
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The effect of aminoacylation on the conformation of yeast tRNAPhe was investigated by high-resolution (300 MHz) proton nuclear magnetic resonance (NMR) spectroscopy. Resonances in the low-field (-11 to -15 ppm) region of the spectra are due to ring NH protons of Watson-Crick base pairs, and to a very high degree of approximation (within 0.05 ppm) the low-field spectra of tRNAPhe and phenylalanyl-tRNAPhe are identical. From this observation and analysis of the low-field NMR spectra we conclude that the secondary structures of the two tRNAs are identical with respect to base-pairing schemes and interbase distances in the helical region (0.1-0.2 A). Several tertiary structural features, including conformation of the dihydro-U loop, conformation of the minor loop, relative orientations of the acceptor and the T{Psi}C stems, dihydro-U and anticodon stems, and probably conformation of the anticodon loop are shown to be the same in tRNAPhe and phenylalanyl-tRNAPhe. Our results leave little remaining opportunity for changes in tertiary structure that would not have been observed by the NMR method.
关键词：base-pairing ; 300 MHz proton nuclear magnetic resonance
