期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：8
页码：2330-2334
DOI：10.1073/pnas.70.8.2330
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Extracts prepared from commercially available wheat germ efficiently translate messenger RNAs from either viral or eukaryotic origin. The addition of tobacco mosaic virus RNA stimulated amino-acid incorporation more than 100-times and rabbit globin 9S RNA between 20- and 30-times. The in vitro product directed by globin 9S RNA comigrated precisely with authentic rabbit globin on sodium dodecyl sulfate-polyacrylamide gels. Furthermore, during high voltage ionophoresis two [35S]methionine-labeled tryptic peptides synthesized in vitro comigrated in two dimensions with [α]T5 and {beta}T5 tryptic peptides from authentic [35S]methionine-labeled rabbit globin.
关键词：wheat germ extracts ; protein synthesis ; gel electrophoresis ; peptide analysis
