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  • 标题:Lateral Phase Separations in Membrane Lipids and the Mechanism of Sugar Transport in Escherichia coli
  • 本地全文:下载
  • 作者:C. D. Linden ; K. L. Wright ; H. M. McConnell
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1973
  • 卷号:70
  • 期号:8
  • 页码:2271-2275
  • DOI:10.1073/pnas.70.8.2271
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Changes in slope of Arrhenius plots for transport can, in some instances, be detected at two different temperatures for cells that have a relatively simple fatty-acid composition in the membrane lipids. These characteristic temperatures correlate with the characteristic temperatures that define changes of state in membrane phospholipids as revealed by the paramagnetic resonance of the spin label TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl). The higher of these characteristic temperatures is that at which the formation of solid patches of membrane lipids is first detected. The lower is the end point of the course of lateral phase separations, at which all the membrane lipids are in a solid phase. For cells enriched for elaidic acid, the rate of transport increase by as much as 2-fold as the temperature is decreased by less than 1{degrees
  • 关键词:β-oxidationless fatty-acid auxotrophs ; spin labels
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