期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：8
页码：2291-2294
DOI：10.1073/pnas.70.8.2291
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Human parathyroid glands from a patient with chronic renal failure were used to study in vitro biosynthesis of immunoreactive parathyroid hormone. Parathyroid glands were incubated in the presence of [3H]leucine, and classical subcellular fractions were prepared. peptides from acetic acid extractions of the fractions were separated on Sephadex G-75 Superfine. Eluates were assayed for immunoreactive parathyroid hormone by a double-antibody technique. Leucine was incorporated into several large immunoreactive peptides of parathyroid hormone having estimated molecular weights of >25,000, 18,000, and 13,000. The large forms of immunoreactive parathyroid hormone were prominant in the microsomes. The secretion granule fraction, as well as the patient's serum, contained principally immunoreactive parathyroid hormone with an apparent molecular weight of 10,000.
