期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：8
页码：2356-2359
DOI：10.1073/pnas.70.8.2356
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Survival characteristics, after transfusion, of erythrocytes from patients with homozygous sickle-cell disease were studied in rats. The study was made possible by previous injection of the animals with ethyl plamitate, which depressed reticuloendothelial system function, and with a factor in cobra venom that inactivated complement. This treatment prevented the rapid phagocytosis and intravascular hemolysis of donor erythrocytes that usually follow a heterologous transfusion. Both immediate after transfusion recovery and survival of 51Cr-labeled sickle erythrocytes were decreased in comparison to the values obtained for control erythrocytes from individuals without sickle-cell anemia. Survival of sickle erythrocytes was improved during exposure of the animals to 100% O2. Hypoxia (7-10% O2) resulted in the abrupt removal of 35-60% of sickle erythrocytes from the rats' circulation. Variations in oxygen tension did not affect survival of control erythrocytes. The usefulness of this convenient animal model for the study of sickle-cell anemia is suggested by the similarities between our results and the behavior of sickle erythrocytes in humans. The system may also be suitable for studying a wide variety of other human erythrocyte disorders.
