期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：9
页码：2600-2604
DOI：10.1073/pnas.70.9.2600
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Eukaryotic cytoplasmic initiator tRNAs lack the sequence G-T-{Psi}-C-G(A)-, which is in every tRNA of known sequence that is active in protein biosynthesis. In initiator tRNA of yeast cytoplasm, which is the only eukaryotic initiator tRNA of known sequence, this sequence is replaced by G-A-U-C-G-. We now report the sequence of a 30-nucleotide-long, 3'-terminal fragment of cytoplasmic initiator tRNA of rabbit liver obtained by specific cleavage of the tRNA at the site occupied by the modified nucleoside, 7-methyl guanosine. We show (i) that in rabbit-liver initiator tRNA also, the sequence G-T-{Psi}-C-G(A)- is replaced by G-A-U-C-G- and (ii) that the sequences of loop IV of both the yeast and rabbit-liver cytoplasmic initiator tRNAs are identical, A-U-C-G-m1-A-A-A-.
关键词：eukaryotic initiator tRNA ; fingerprinting ; specific chemical cleavage ; nucleotide sequence analysis
