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  • 标题:Enzyme Assay in Microsomes Below Zero Degrees
  • 本地全文:下载
  • 作者:Pascale Debey ; Claude Balny ; Pierre Douzou
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1973
  • 卷号:70
  • 期号:9
  • 页码:2633-2636
  • DOI:10.1073/pnas.70.9.2633
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Reactions of a membrane-bound multienzyme complex (electron-transport chain of rat-liver microsomes) suspended in aqueous-organic solvent used as antifreeze at temperatures below 0{degrees} were studied. In the presence of a relatively high concentration of ethylene glycol, electron transfer can still be observed and some individual and sequential enzyme assays can be performed over a wide range of temperatures below 0{degrees}.
  • 关键词:ethylene glycol ; electron transfer ; rats ; liver
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