标题:Isolation of Escherichia coli RNA Polymerase Binding Sites on T5 and T7 DNA: Further Evidence for Sigma-Dependent Recognition of A-T-Rich DNA Sequences
期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:1973
卷号:70
期号:10
页码:2911-2915
DOI:10.1073/pnas.70.10.2911
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:Previous isolation and analysis of E. coli RNA polymerase (EC 2.7.7.6 ) binding sites on {lambda} DNA had demonstrated the existence of a sigma-dependent process of recognition of A-T-rich DNA sequences. We have now extended this finding to T5 and T7 DNA and have provided evidence for the double-strandedness of the isolated binding sites. The possible equation of these sites to the genetically defined promoters is discussed.