期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：1
页码：200-204
DOI：10.1073/pnas.71.1.200
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The heterogeneous RNA of the HeLa cell nucleus contains short internal poly(A) sequences which, in contrast to the longer poly(A) sequences at the 3' ends, are not found in messenger RNA of the cytoplasm. A distinct origin for each of these homologous sequences is evident when the effects of actinomycin D and 3'-deoxyadenosine on their biosynthesis are compared. The shorter poly(A) appears to be transcribed, while the longer one does not. A kinetic analysis of the metabolism of each type of poly(A) sequence within different size classes of HnRNA after treatment of the cells with 3'-deoxyadenosine reveals distinctive distribution and metabolism of these sequences and also provides insight into mechanisms proposed for the generation of messenger RNA from these large RNA molecules in the nucleus.
关键词：transcriptional synthesis ; messenger RNA ; 3′-deoxyadenosine ; actinomycin D
