期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：1
页码：99-103
DOI：10.1073/pnas.71.1.99
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The reiteration frequency for mouse immunoglobulin VH-genes and CH-genes has been directly estimated by hybridization of purified MOPC 315 [α]-chain mRNA with a vast excess of mouse DNA. A biphasic Cot curve resulted. The low Cot transition (Cot1/2 about 1.5) was interpreted as hybridization to VH-genes and the high Cot transition (Cot1/2 about 103) as hybridization to CH-genes. These values correspond to about 5000 VH-genes and less than 8 CH-genes. This germ-line content of VH-genes is sufficient to account for antibody diversity given a comparable set of VL-genes. Minimal-gene models are invalidated and there is no need to invoke somatic generators of diversity.
关键词：mouse myeloma ; H-chain mRNA ; variable region ; constant region
